pshp 1 Search Results


pshp1 antibody  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc pshp1 antibody
Western blotting analysis (A) demonstrated significant increase in the level of <t>pVEGF-R2</t> after 5 and 60 minutes from the beginning of treatment with 12-HETE. *P<0.05 vs Control (C) and other time points. There was simultaneous decrease in the level of pSHP1. Immunofluorescence of pSHP1 (green) in REC also showed marked decrease in the pSHP1 immunoreactivity by 12-HETE compared to the control. * P<0.05 vs control (n = 3).
Pshp1 Antibody, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Western blotting analysis (A) demonstrated significant increase in the level of pVEGF-R2 after 5 and 60 minutes from the beginning of treatment with 12-HETE. *P<0.05 vs Control (C) and other time points. There was simultaneous decrease in the level of pSHP1. Immunofluorescence of pSHP1 (green) in REC also showed marked decrease in the pSHP1 immunoreactivity by 12-HETE compared to the control. * P<0.05 vs control (n = 3).

Journal: PLoS ONE

Article Title: 12/15-Lipoxygenase-Derived Lipid Metabolites Induce Retinal Endothelial Cell Barrier Dysfunction: Contribution of NADPH Oxidase

doi: 10.1371/journal.pone.0057254

Figure Lengend Snippet: Western blotting analysis (A) demonstrated significant increase in the level of pVEGF-R2 after 5 and 60 minutes from the beginning of treatment with 12-HETE. *P<0.05 vs Control (C) and other time points. There was simultaneous decrease in the level of pSHP1. Immunofluorescence of pSHP1 (green) in REC also showed marked decrease in the pSHP1 immunoreactivity by 12-HETE compared to the control. * P<0.05 vs control (n = 3).

Article Snippet: Retina homogenates were reacted with primary antibodies against NOX2 or gp91 phox (mouse monoclonal, 1 500, BD transduction laboratories, San Diego, CA), ZO-1, 2 μg/ml (Invitrogen, Eugene, OR), ICAM-1 (1 250, Santa Cruz, Santa Cruz, CA), pVEGF-R2, VEGF-R2 (1 250, Abcam, Cambridge, MA) and pSHP1 (1 200, Stem Cell Technologies, Vancouver, Canada).

Techniques: Western Blot, Control, Immunofluorescence

There was marked increase in the pVEGF immunoreactivity in retinal sections from diabetic mice in comparison to the control. This was prevented by baicalein treatment (A). Note that pVEGF-R2 expression was localized mainly in Muller cells in the control (long arrows), however the reaction was also increased in retinal vessels during diabetes (short arrows) * P<0.05 vs control and # P<0.05 vs baicalein treated group (n = 5). pSHP immunolocalization (B) revealed marked decrease in diabetes which was restored by baicalein treatment. Note that pSHP is expressed in different retinal layers and also in relation to retinal vessels (arrows) in normal retina. In diabetes the decrease in pSHP occurred mainly in inner retina including retinal vessels. * P<0.05 vs control and # P<0.05 vs baicalein treated group (n = 5).

Journal: PLoS ONE

Article Title: 12/15-Lipoxygenase-Derived Lipid Metabolites Induce Retinal Endothelial Cell Barrier Dysfunction: Contribution of NADPH Oxidase

doi: 10.1371/journal.pone.0057254

Figure Lengend Snippet: There was marked increase in the pVEGF immunoreactivity in retinal sections from diabetic mice in comparison to the control. This was prevented by baicalein treatment (A). Note that pVEGF-R2 expression was localized mainly in Muller cells in the control (long arrows), however the reaction was also increased in retinal vessels during diabetes (short arrows) * P<0.05 vs control and # P<0.05 vs baicalein treated group (n = 5). pSHP immunolocalization (B) revealed marked decrease in diabetes which was restored by baicalein treatment. Note that pSHP is expressed in different retinal layers and also in relation to retinal vessels (arrows) in normal retina. In diabetes the decrease in pSHP occurred mainly in inner retina including retinal vessels. * P<0.05 vs control and # P<0.05 vs baicalein treated group (n = 5).

Article Snippet: Retina homogenates were reacted with primary antibodies against NOX2 or gp91 phox (mouse monoclonal, 1 500, BD transduction laboratories, San Diego, CA), ZO-1, 2 μg/ml (Invitrogen, Eugene, OR), ICAM-1 (1 250, Santa Cruz, Santa Cruz, CA), pVEGF-R2, VEGF-R2 (1 250, Abcam, Cambridge, MA) and pSHP1 (1 200, Stem Cell Technologies, Vancouver, Canada).

Techniques: Comparison, Control, Expressing